Opportunity Information: Apply for G23AS00341

This funding opportunity (G23AS00341) is a discretionary cooperative agreement from the US Geological Survey (USGS) under the Cooperative Ecosystem Studies Unit (CESU) framework, specifically tied to the Rocky Mountain CESU network. The project focuses on the genetics of the Oregon spotted frog (Rana pretiosa), a threatened amphibian found only in the Pacific Northwest. The central purpose is to generate actionable genetic and genomic information that directly supports priorities laid out in the US Fish and Wildlife Service (USFWS) Recovery Plan for the species, helping agencies make better-informed conservation and recovery decisions.

The scientific emphasis is on applying modern genomic approaches rather than the older genetic tools that have been used in prior Oregon spotted frog studies. Historically, research on this species relied on allozymes and microsatellite markers, which were standard before the recent advances in genomics. In contrast, current high-throughput sequencing methods can generate information from thousands to tens of thousands of genomic loci, providing much finer resolution for understanding how populations are structured, how connected or isolated they are, and whether there are meaningful adaptive differences among groups. In practical terms, the work is intended to delineate conservation units (groups that may need to be managed separately), quantify connectivity among populations (gene flow and dispersal patterns), and estimate a suite of population genetic parameters that indicate risk and resilience, such as genetic diversity, differentiation, and signs of isolation.

The conservation need behind the project is clear: Oregon spotted frogs have declined across much of their range due to wetland loss, degradation, and fragmentation. These habitat changes are linked to altered hydrology as well as the spread of invasive vegetation and predators, which collectively reduce habitat quality and isolate populations. Because fragmented and declining populations are especially vulnerable to inbreeding, loss of genetic variation, and reduced ability to adapt to environmental change, improved genetic and genomic information is a key missing piece for evaluating threats and selecting recovery actions that are likely to work. The USGS notes that this effort is part of broader coordination with the USFWS to better understand amphibian distribution, demography, and responses to restoration and management actions aimed at stabilizing and recovering Oregon spotted frog populations.

Administratively, this opportunity is only open to eligible applicants that are official participating partners of the Rocky Mountain CESU Program. CESUs are partnership networks designed to streamline and support collaborative research, technical assistance, and education among federal agencies and partner institutions. The award is offered as a cooperative agreement, which typically indicates substantial involvement and collaboration with the funding agency during project execution, rather than a more hands-off grant structure.

Key details from the notice include: the funding activity category is Science and Technology and other Research and Development; the CFDA number is 15.808; the agency is the US Geological Survey; the opportunity was created on 2023-04-20 with an original closing date of 2023-05-22; and the listed award ceiling is $84,773.

  • The Geological Survey in the science and technology and other research and development sector is offering a public funding opportunity titled "Cooperative Agreement for CESU-affiliated Partner with Rocky Mountain Cooperative Ecosystem Studies Unit" and is now available to receive applicants.
  • Interested and eligible applicants and submit their applications by referencing the CFDA number(s): 15.808.
  • This funding opportunity was created on 2023-04-20.
  • Applicants must submit their applications by 2023-05-22. (Agency may still review applications by suitable applicants for the remaining/unused allocated funding in 2026.)
  • Each selected applicant is eligible to receive up to $84,773.00 in funding.
  • Eligible applicants include: Others.
Apply for G23AS00341

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Frequently Asked Questions (FAQs)

What is the funding opportunity number for this award?

The funding opportunity number is G23AS00341.

Which federal agency is offering this opportunity?

This opportunity is offered by the U.S. Geological Survey (USGS).

What type of funding instrument is being used?

The award is a discretionary cooperative agreement.

What does it mean that this is a cooperative agreement?

A cooperative agreement generally indicates substantial involvement and collaboration by the funding agency during the project period, rather than a more hands-off relationship typical of some grant awards.

What program framework is this opportunity associated with?

This opportunity is offered under the Cooperative Ecosystem Studies Unit (CESU) framework and is specifically tied to the Rocky Mountain CESU network.

Who is eligible to apply?

Eligibility is limited to applicants that are official participating partners of the Rocky Mountain CESU Program.

Is this opportunity open to organizations outside the Rocky Mountain CESU partnership?

No. Based on the notice, the opportunity is only open to eligible applicants that are official participating partners of the Rocky Mountain CESU Program.

What is the main focus of the project?

The project focuses on the genetics and genomics of the Oregon spotted frog (Rana pretiosa), a threatened amphibian found only in the Pacific Northwest.

What is the central purpose of the work being funded?

The central purpose is to generate actionable genetic and genomic information that directly supports priorities in the U.S. Fish and Wildlife Service (USFWS) Recovery Plan for the Oregon spotted frog, helping agencies make better-informed conservation and recovery decisions.

Which species is being studied?

The species is the Oregon spotted frog (Rana pretiosa).

What is the conservation status of the Oregon spotted frog as described in the notice?

The notice describes the Oregon spotted frog as a threatened amphibian.

Where is the Oregon spotted frog found?

The notice states the Oregon spotted frog is found only in the Pacific Northwest.

Which recovery plan is this project intended to support?

The project is intended to support priorities laid out in the USFWS Recovery Plan for the Oregon spotted frog.

What scientific approaches does this project emphasize?

The project emphasizes modern genomic approaches, including high-throughput sequencing methods, rather than older genetic tools used in prior studies.

Which older genetic tools are mentioned as having been used historically for this species?

Historically, research on Oregon spotted frogs relied on allozymes and microsatellite markers.

Why does the notice emphasize modern genomics over older methods?

The notice explains that high-throughput sequencing can generate information from thousands to tens of thousands of genomic loci, providing much finer resolution for understanding population structure, connectivity or isolation, and potential adaptive differences among groups.

What kinds of conservation questions is the genomic work intended to answer?

The work is intended to help delineate conservation units, quantify connectivity among populations (including gene flow and dispersal patterns), and estimate population genetic parameters related to risk and resilience.

What are "conservation units" in the context of this opportunity?

In this notice, conservation units refer to groups of frogs that may need to be managed separately based on meaningful genetic or genomic differences.

What does "connectivity among populations" refer to here?

Connectivity refers to how connected or isolated populations are, including gene flow and dispersal patterns.

What population genetic parameters are specifically mentioned?

The notice mentions genetic diversity, differentiation, and signs of isolation as examples of parameters used to indicate risk and resilience.

Why is improved genetic and genomic information considered important for this species?

The notice links population fragmentation and decline to increased vulnerability to inbreeding, loss of genetic variation, and reduced ability to adapt to environmental change. Improved genetic and genomic information is described as a key missing piece for evaluating threats and selecting effective recovery actions.

What threats or drivers of decline are described for Oregon spotted frogs?

The notice states the species has declined due to wetland loss, degradation, and fragmentation, and ties these changes to altered hydrology as well as the spread of invasive vegetation and predators.

How do habitat changes affect Oregon spotted frog populations according to the notice?

The notice explains that habitat changes reduce habitat quality and isolate populations, which can increase genetic risks for fragmented and declining populations.

How does this project relate to other agency efforts?

USGS notes the effort is part of broader coordination with USFWS to better understand amphibian distribution, demography, and responses to restoration and management actions aimed at stabilizing and recovering Oregon spotted frog populations.

What is the funding activity category listed for this opportunity?

The funding activity category is Science and Technology and other Research and Development.

What is the CFDA number associated with this opportunity?

The CFDA number listed is 15.808.

What is the award ceiling listed in the notice?

The listed award ceiling is $84,773.

When was this opportunity created?

The notice lists the creation date as 2023-04-20.

What was the original closing date for applications?

The notice lists an original closing date of 2023-05-22.

Does the notice indicate that the work must directly support agency decision-making?

Yes. The notice frames the goal as producing actionable genetic and genomic information that directly supports recovery plan priorities and helps agencies make better-informed conservation and recovery decisions.

Does the notice specify that the project is intended to replace prior genetic studies?

The notice does not state the project replaces prior work, but it emphasizes that older tools (allozymes and microsatellites) were used historically and that modern high-throughput genomic methods offer much finer resolution for current conservation needs.

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